بررسی اثر ضد میکروبی شقایق کوهی (Glaucium) بر میکرو فلور دهان

Normal 0 false false false EN-US X-NONE AR-SA In this study, 4 species of Glaucium (Corniculatum, Elegans, Flavum and fimberiligerm) was used and their root, stem, flower, leaf and seeds were dried and grinded. Then, the extract was made by adding ethanol, methanol and water. By using sterile swab, oral microbial flora of 10000 patients were taken and cultivated on different agar cultures. The microbial strains involved: streptococci, bacillus, actinomycetes, diphteroids, lactobacillus and candida. Antimicrobial effect was evaluated in various media by measuring the diameter of microbial zone and compare with control group. The antimicrobial effect of the studied species on total count of oral microbial flora and also on each bacterial species was recorded.  Acetyl pyridinum chloride was used in control group. The amount of absorbed extract was 1.25 mg in each blank disc. The different concentrations of 1.25, 2.5, 5 and 10 were prepared and analyzed. The results showed all 4 species had antimicrobial effect and the least effective concentration was 2.5%. The Elegans specie was more effective than the others. Muler-Hinton medium was the most suitable media among the others. The extracts of ethanol, methanol, water and autoclaved showed the most to the least antimicrobial effect respectively.  The antimicrobial effect was related to the specific oral microflora of every individual. Streptococci were the most sensitive while bacillus was the least sensitive bacteria to the extract. /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal" mso-tstyle-rowband-size:0 mso-tstyle-colband-size:0 mso-style-noshow:yes mso-style-priority:99 mso-style-qformat:yes mso-style-parent:"" mso-padding-alt:0cm 5.4pt 0cm 5.4pt mso-para-margin-top:0cm mso-para-margin-right:0cm mso-para-margin-bottom:10.0pt mso-para-margin-left:0cm line-height:115% mso-pagination:widow-orphan font-size:11.0pt font-family:"Calibri","sans-serif" mso-ascii-font-family:Calibri mso-ascii-theme-font:minor-latin mso-hansi-font-family:Calibri mso-hansi-theme-font:minor-latin mso-bidi-font-family:Arial mso-bidi-theme-font:minor-bidi}